Determination of Handedness of DNA Double Helices From NOE Difference Spectra: The Structure of Poly(dG-dC)•Poly(dG-dC) in Low Salt
- 1 October 1983
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 1 (2), 417-428
- https://doi.org/10.1080/07391102.1983.10507451
Abstract
Combined use of shielding constant computations, measurements of chemical shifts and NOE studies reveal that poly(dG-dC)•(poly)dG-dC) in low salt solutions exist as a right- handed B-DNA double helix described by Gupta, Dhingra, Sarma, Sarma, Rajagopalan and Sasisekharan, J. Biomole. Str. Dyn. 1, 395, 1983. We present a simple and direct method to determine the handedness of DNA double helices from NOE difference spectra. This method takes advantage of the NOE between base protons and the H2′H2” sugar protons; and in the difference NOE spectra in the H2′H2” region the signatures of the right and left-handed helices become imprinted.This publication has 15 references indexed in Scilit:
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