Detection of IgG Antibodies to Type-Specific Pseudomonas aeruginosa Lipopolysaccharides by Solid-Phase Radioimmunoassay

Abstract

Previous studies have suggested that IgG serotype-specific antibodies are protective against infections with Pseudomonas aeruginosa. In the present study, type-specific IgG antibodies to P. aeruginosa were detected by solid-phase radioimmunoassay in sera from 15 volunteers before and after vaccination with lipopolysaccharides from P. aeruginosa and from four patients with endocarditis due to P. aeruginosa. Significant type-specific increases in IgG antibody occurred after both vaccination and infection. The correlation coefficients comparing net counts per minute by solidphase radioimmunoassay with hemagglutination titers in the 15 vaccinees were 0.940, 0.874, 0.792, 0.903, 0.882, 0.869, and 0.704 for serotypes 1–7, respectively.