THE EXTRACTION AND DETERMINATION OF 17′KETOSTEROIDS IN URINE1
- 1 September 1940
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 27 (3), 355-359
- https://doi.org/10.1210/endo-27-3-355
Abstract
A modification of current methods for extraction of 17-ketosteroids from urine was described. 500 cc. of urine were hydrolyzed by steam for 20 mins. Extraction was performed in a continuous extractor which comprises an extraction column, a conduit for the solvent vapor and an automatic collecting tube for returning the extract to the flask. The extractor was illustrated and described in the original article. CCI4 was used as the solvent and the extraction was complete within 60 mins. The use of ether was eliminated. Recoveries by this method were approx. 100%. From 12-16 complete analyses might be performed by 1 person in 1 day with 2 units.This publication has 2 references indexed in Scilit:
- A RAPID EXTRACTOR FOR URINARY ANDROGENS FACTORS TO BE CONSIDERED IN THE PREPARATION OF EXTRACTS FOR COLORIMETRIC ASSAY11Endocrinology, 1939
- Estimation of (capon) comb growth hormone in urineBiochemical Journal, 1938