Autointerference of Rabies Virus in Chick Embryo Fibroblasts.

Abstract

Summary When HEP Flury strain of rabies virus was inoculated onto chick embryo fibroblasts at a multiplicity of higher than 0.1, no plaques were formed and almost all cells remained stainable with neutral red. Monolayers pretreated with such a concentration of HEP Flury virus showed interference with plaque formation by vaccinia and WEE viruses. This was not due to presence of interferon in the seed virus suspension, nor to the presence of tissue components in the inoculum. In bottle cultures of chick embryo cells, virus growth after infection at higher multiplicities was accompanied by production of a considerable amount of interferon, and the highest virus yield was obtained when the multiplicity of infection was about 0.01.