RAD50 protein ofS.cerevisiaeexhibits ATP-dependent DNA binding
- 11 August 1993
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 21 (16), 3851-3856
- https://doi.org/10.1093/nar/21.16.3851
Abstract
RAD50 function of Saccharomyces cerevisiae is required during vegetative growth for recombinational repair of DNA double strand breaks, and during meiosis for initiation of meiotic recombination and formation of synaptonemal complex. RAD50 encodes a 153 kDa polypeptide which includes an amino-terminal ATP binding domain essential for function and two long heptad repeat regions. We show below that RAD50 protein purified from yeast exhibits ATP-dependent binding to double stranded DNA. Physical properties of the purified protein are also described. Models for RAD50 function in vivo are discussed.Keywords
This publication has 29 references indexed in Scilit:
- Expression of the Saccharomyces cerevisiae RAD50 gene during meiosis: steady-state transcript levels rise and fall while steady-state protein levels remain constantMolecular Genetics and Genomics, 1993
- Protein volumes and hydration effects. The calculations of partial specific volumes, neutron scattering matchpoints and 280-nm absorption coefficients for proteins and glycoproteins from amino acid sequencesEuropean Journal of Biochemistry, 1986
- Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotinNucleic Acids Research, 1985
- Conformational states of smooth muscle myosin. Effects of light chain phosphorylation and ionic strength.Journal of Biological Chemistry, 1984
- A bent monomeric conformation of myosin from smooth muscle.Proceedings of the National Academy of Sciences, 1982
- Recombinationless meiosis in Saccharomyces cerevisiae.Molecular and Cellular Biology, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Three Forms of the 5.8‐S Ribosomal RNA Species in Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductasesBiochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis, 1966