Analysis of Biological Fluids for Carbon Tetrachloride after its Administration into the Bovine Rumen

Abstract

The transmission of 4g of CCl4 from the rumen of a dairy cow into her blood, milk, and urine, was monitored by chromatographic analysis of head space vapors of these biological fluids. The CCI4 was observed in milk, blood, and urine 15 minutes after its administration. Its concentration in these fluids increased gradually during the first hour, reached peaks of 3.35ppm in the blood after the first day and 6.9 and 1.8ppm in the milk and urine, respectively, after the 2nd day and returned to near normal on the 3rd day. Changes in the normal concentration of acetone in milk, blood, and urine were also observed in the CCl4 treated cow. Acetone reached maximum concentrations (urine 2.85ppm, milk 2.8ppm, blood 2.65ppm) one day after administration of CCl4 and did not return to near normal (about l.Oppm) until the 3rd day. This technique is suggested for studying the transmission of flavors to milk.