Nachweis gentechnologisch ver nderter Pflanzen mittels der Polymerase Kettenreaktion (PCR) am Beispiel derFlavr Savr TM-Tomate
- 1 November 1995
- journal article
- abstracts
- Published by Springer Nature in Zeitschrift für Lebensmittel-Untersuchung und Forschung
- Vol. 201 (6), 583-586
- https://doi.org/10.1007/bf01201590
Abstract
In this study, a practical approach for the detection of a genetically engineered tomato is demonstrated by polymerase chain reaction (PCR) assays. TheFlavr Savr TM tomato available on the market which was used as an example, contains a resistance gene for the antibiotic kanamycin (kansur) and a gene construct for the inhibition of fruit ripening and softening (antisense polygalacturonase). The presence of these DNA's could only be detected in the genetically engineered tomato.This publication has 15 references indexed in Scilit:
- Polymerase chain reaction (PCR) for detection of pathogenic microorganisms in bacteriological monitoring of dairy productsResearch in Microbiology, 1995
- Polymerase chain reaction (PCR): a possible alternative to immunochemical methods assuring safety and quality of food Detection of wheat contamination in non-wheat food productsZeitschrift für Lebensmittel-Untersuchung und Forschung, 1993
- Increased Resistance to Potato Virus X and Preservation of Cultivar Properties in Transgenic Potato Under Field ConditionsBio/Technology, 1992
- Reduction of polygalacturonase activity in tomato fruit by antisense RNAProceedings of the National Academy of Sciences, 1988
- Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoesNature, 1988
- Structure and evolution of the intergenic region in a ribosomal DNA repeat unit of wheatJournal of Molecular Biology, 1988
- Primer-directed enzymatic amplification of DNA with a thermostable DNA polymeraseScience, 1988
- Molecular characterization of tomato fruit polygalacturonaseMolecular Genetics and Genomics, 1987
- Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5Gene, 1982
- The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun sequencingNucleic Acids Research, 1981