Nuclease mapping of the secondary structure of the 49-nucleotide 3' terminal cloacin fragment ofEscherichia coli16S RNA and its interactions with initiation factor 3
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (7), 2035-2052
- https://doi.org/10.1093/nar/11.7.2035
Abstract
Escherichia coli translational initiation factor 3 (IF3) may be crosslinked to the 3' end of 16S RNA in 30S ribosomal subunits. In order to determine the sequence to which IF3 may bind in vivo, samples of 5'-32P labelled 3' terminal 49-nucleotide fragment of 16S RNA were incubated 5 min. at 37 degrees in 40 mM Tris-HOAc, pH 7.4, 100 mM NaCl, 1 mM Mg (OAc)2, 1 mM ZnSO4, with or without IF3, then reacted a further 5 min with nuclease S1, RNase T1, or RNase A. Base pairing between the 5' and 3' legs of the fragment occurs in the absence of IF3, but is disrupted by IF3 binding. IF3 appears to protect some residues near the 5' end of the fragment (U1495, A1499, A1500, A1502, and A1503) from nuclease S1, and potentiates S1 attack on others (G1494, G1497, C1501, G1504, G1505, U1506, G1517, G1529, G1530, and C1533). A series of equimolar reactions at increasing dilution imply an association constant range of 1.4-7.0 X 10(7) M-1.Keywords
This publication has 43 references indexed in Scilit:
- The topographical localization of IF3 on Escherichia coli 30 S ribosomal subunits as a clue to its way of functioningFEBS Letters, 1982
- Physical parameters ofEscherichia colitranslational initiation factor 3 binding to poly(A)FEBS Letters, 1981
- The relationship between the the 3′-end of 16 S RNA and the binding of initiation factor if-3 to 30 subunit ofE. ColiFEBS Letters, 1978
- On the relationship between the binding of ribosomal protein S1 to the 30 S subunit of Escherichia coli and 3′ terminus of 16 S RNAJournal of Molecular Biology, 1978
- The primary structure of the initiation factor IF‐3 from Escherichia coliFEBS Letters, 1977
- Protection of specific sites in 16 S RNA from chemical modification by association of 30 S and 50 S ribosomesJournal of Molecular Biology, 1977
- The 3′‐terminus of 16 S ribosomal RNA of Escherichia coli. Isolation and purification of the terminal 49‐nucleotide fragment at a milligram scaleFEBS Letters, 1976
- Cross-linking of initiation factor IF3 to proteins of the Escherichia coli 30 S ribosomal subunitJournal of Molecular Biology, 1976
- Near neighbors of IF3 bound to 30S ribosomal subunitsFEBS Letters, 1975
- 30S Ribosomal proteins associated with the 3′‐terminus of 16S RNAFEBS Letters, 1975