3,4‐Dichloroisocoumarin, a serine protease inhibitor, inactivates glycogen phosphorylase b
- 30 July 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 268 (1), 133-136
- https://doi.org/10.1016/0014-5793(90)80991-q
Abstract
3,4‐Dichloroisocoumarin (3,4‐DCI) is a highly reactive, mechanism‐based inhibitor of serine proteases. We show here that glycogen phosphorylase b is also inactivated by this inhibitor, in a mechanism that parallels the inactivation of serine proteases, but involving multiple sites of covalent modification. Such a process may compromise studies in which 3,4‐DCI is used to arrest proteolysis of a second native protein which may itself be modified.Keywords
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