P. M. R. Spectroscopic Analysis of Bacterial Polysaccharides Containing Pyruvic Acid
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 5 (10), 675-681
- https://doi.org/10.1080/00032717208064348
Abstract
The characteristic p.m.r. signal at τ 8.4–8.6 given by the CH3-C group of a pyruvic acid ketal (1-carboxyethylidene derivative)may be used to demonstrate the presence of such a substituent in polysaccharides. Similar signals are given when the pyruvic acid ketal is methylated or reduced. The method is convenient for monitoring purification procedures and chemical transformations of polysaccharides such as are involved in structural studies. In certain cases the molar ratio of pyruvic acid may be estimated.Keywords
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