ISGF3, the transcriptional activator induced by interferon alpha, consists of multiple interacting polypeptide chains.
- 1 November 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (21), 8555-8559
- https://doi.org/10.1073/pnas.87.21.8555
Abstract
Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.This publication has 31 references indexed in Scilit:
- Cytoplasmic activation of ISGF3, the positive regulator of interferon-alpha-stimulated transcription, reconstituted in vitro.Genes & Development, 1989
- IκB: a Specific Inhibitor of the NF-κB Transcription FactorScience, 1988
- Interferon-induced nuclear factors that bind a shared promoter element correlate with positive and negative transcriptional control.Genes & Development, 1988
- Human CCAAT-binding proteins have heterologous subunitsCell, 1988
- Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-κB transcription factorCell, 1988
- Interferon-induced transcription of a gene encoding a 15-kDa protein depends on an upstream enhancer element.Proceedings of the National Academy of Sciences, 1987
- Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.Proceedings of the National Academy of Sciences, 1987
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- [36] Eukaryotic gene transcription with purified componentsMethods in Enzymology, 1983
- Epidermal growth factor rapidly stimulates prolactin gene transcriptionNature, 1982