SYNTHESIS OF CARBOXYL TERMINAL SEGMENTS OF BETA‐SUBUNIT OF HUMAN CHORIONIC GONADOTROPIN

Abstract

The synthesis in solution of carboxyl terminal peptide segments of the beta-subunit of human chorionic gonadotropin is described. The protected segments include sequences 119–131, 132–137, and 138–145. The syntheses were based on a standardized liquid-liquid extraction program for routine purification of intermediates (two-phase method). Condensation of terminally deblocked segments afforded protected peptides 132–145, 126–145, 120–145 and 119–145. Protected peptides 126–145 and 120–145 were deprotected in liquid hydrogen fluoride and used in conjugated form for immunization of rabbits. Data on the specificity of the antibody response are reported.