Pregnancies following the frozen storage of expanding human blastocysts
- 1 June 1985
- journal article
- research article
- Published by Springer Nature in Journal of Assisted Reproduction and Genetics
- Vol. 2 (2), 59-64
- https://doi.org/10.1007/bf01139337
Abstract
Human blastocysts were frozen in Earle's solution containing pyruvate and human serum, using glycerol as cryoprotectant, and stored in liquid nitrogen. Thawed blastocysts were replaced in 11 patients, which resulted in two pregnancies. One blastocyst giving a pregnancy was hatching when replaced. Three parameters appeared to be important for embryo survival and implantation: the interval between ovulation and replacement of the thawed blastocysts, satisfactory embryonic development before freezing, and the stage of blastulation when cooling began.Keywords
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