Enzyme degradation, high performance liquid chromatography and liquid secondary ion mass spectrometry in the analysis of glycoproteins
- 1 November 1990
- journal article
- research article
- Published by Wiley in Biomedical Chromatography
- Vol. 4 (6), 261-266
- https://doi.org/10.1002/bmc.1130040612
Abstract
Analysis of small amounts of glycoproteins by high performance liquid chromatography (HPLC) and liquid secondary ion mass spectrometry (LSIMS) together with enzyme digestion has been investigated using fetuin as a model. Preliminary data indicates that 71% of the expected peptides were detected by LSIMS analysis of 200 pmol total digest. HPLC profiles of peptides and glycopeptides were obtained from 2 nmol of digest using a reversed phase (C18) column eluted in a solvent system containing TFA, water and acetonitrile. This has provided glycopeptides for subsequent oligosaccharide analysis. Strategies are reviewed for the chromatographic characterization of oligosaccharides following their release from glycopeptides by chemical and enzymatic procedures.Keywords
This publication has 49 references indexed in Scilit:
- Microscale sequencing of O‐linked oligosaccharides using mild periodate oxidation of alditols, coupling to phospholipid and TLC‐MS analysis of the resulting neoglycolipidsEuropean Journal of Biochemistry, 1990
- Separation of branched sialylated oligosaccharides using high-pH anion-exchange chromatography with pulsed amperometric detectionAnalytical Biochemistry, 1989
- The effect of alkaline borohydride treatment onN-linked carbohydrates of glycoproteinsGlycoconjugate Journal, 1989
- NEW TYPE OF ADHESIVE SPECIFICITY REVEALED BY OLIGOSACCHARIDE PROBES IN ESCHERICHIA COLI FROM PATIENTS WITH URINARY TRACT INFECTIONThe Lancet, 1988
- Hydrophobic effects in the fast atom bombardment mass spectra of proteins and large peptidesJournal of Mass Spectrometry, 1988
- Binding of N-linked bovine fetuin glycopeptides to isolated rabbit hepatocytes: Gal/GalNAc hepatic lectin discrimination between Gal.beta.(1,4)GlcNAc and Gal.beta.(1,3)GlcNAc in a triantennary structureBiochemistry, 1986
- Asparagine-linked sugar chains of fetuin: occurrence of tetrasialyl triantennary sugar chains containing the Gal.beta.1 .fwdarw. 3GlcNAc sequenceBiochemistry, 1986
- Novel approach to the study of the antigenicities and receptor functions of carbohydrate chains of glycoproteinsBiochemical and Biophysical Research Communications, 1985
- Mild alkaline borohydride treatment liberates N‐acetylglucosamine‐linked oligosaccharide chains of glycoproteinsFEBS Letters, 1981
- The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharidesBiochimica et Biophysica Acta (BBA) - General Subjects, 1970