Isolation and characterization of alphoid DNA sequences specific for the pericentric regions of chromosomes 4, 5, 9, and 19
- 1 January 1988
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 47 (3), 144-148
- https://doi.org/10.1159/000132533
Abstract
We have cloned and characterized two distinct types of alphoid DNA elements. Probe pG-Xbal 1/340 was obtained by random cloning of human satellite DNA and contains two basic units with overall 88% homology to the 171 -bp consensus alphoid sequence. pG-Xball/340-like elements are represented about 2,000-4,000 times in the haploid genome and, by in situ hybridization, are found exclusively at the primary constrictions of chromosomes 4 and 9. Probe pG-A16 was cloned from a chromosome 19-specific cosmid library and represents a 2.25-kb higher-order DNA element which is present at roughly 75-150 copies per haploid genome and which hybridizes to the centromeres of chromosomes 5 and 19. Using the pG-A16 probe, further genetic and physical dissection of the central area of chromosome 19 can be envisaged.Keywords
This publication has 5 references indexed in Scilit:
- Chromosomal location by in situ hybridization of the human Sau3A family of DNA repeatsHuman Genetics, 1987
- Detection of restriction fragment length polymorphisms at the centromeres of human chromosomes by using chromosome-specific alpha satellite DNA probes: implications for development of centromere-based genetic linkage maps.Proceedings of the National Academy of Sciences, 1986
- Dideoxy sequencing method using denatured plasmid templatesAnalytical Biochemistry, 1986
- Toward early diagnosis of myotonic dystrophy:construction and characterization of a somatic cell hybrid with a single human der(19) chromosomeCytogenetic and Genome Research, 1986
- Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells.Journal of Clinical Pathology, 1985