THE EFFECTS OF 4-AMINO-N10-METHYL-PTEROYLGLUTAMIC ACID ON THE LEUKOCYTES OF THE NORMAL AND LEUKEMIC MOUSE

Abstract
4-Amino-N10-methyl-pteroylglutamic acid in the usual treatment dosage caused a slight leukopenia, but no change in the differential count of normal mice. However, in animals with the Ak 4 strain of transplanted leukemia, such dosage caused prolongation of survival time, held the total leukocyte count at approximately normal levels, decreased the percentage of abnormal cells, and caused a marked delay in the appearance of leukemic infiltrations in liver, spleen, lymph nodes, kidney and bone marrow. Less than twenty hours after administration, a single massive dose of this drug caused a fall in the total leukocyte count of mice with advanced Ak 4 leukemia.