Sensitive Whole-Cell Biosensor Suitable for Detecting a Variety of N -Acyl Homoserine Lactones in Intact Rhizosphere Microbial Communities
- 1 June 2007
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (11), 3724-3727
- https://doi.org/10.1128/aem.02187-06
Abstract
To investigate quorum sensing in rhizosphere soil, a whole-cell biosensor, Agrobacterium tumefaciens(pAHL-Ice), was constructed. The biosensor responded to all N-acyl homoserine lactones (AHLs) tested, except C(4) homoserine lactone, with a minimum detection limit of 10(-12) M, as well as to both exogenously added AHLs and AHL-producing bacterial strains in soil. This highly sensitive biosensor reveals for the first time the increased AHL availability in intact rhizosphere microbial communities compared to that in bulk soil.Keywords
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