The hydrocarbons of pig liver

Abstract

The un-saponifiable matter from 63 kgm. pig liver was prepared and the cholesterol quantitatively removed. The sterol-free oil obtained (52 gm.) was divided by exhaustive treatment with ether-methyl alcohol into an insoluble fraction, 25.5 gm. of I No. 266, and a soluble fraction, 24.8 gm. of I No. 140. 70% of the insoluble fraction was the unsaturated hydrocarbon of I No. 333, which was shown to be present by Channon and Marrian. The bromide and hydrochloride of this hydrocarbon were prepared in pure form and analyzed. By an adsorption technic the hydrocarbon was isolated in pure form. The analytical data suggest the formula C45H76 or C50H84. Attempts to distill the compound at 10-5 mm. resulted in marked change. Ca. 10% of the insoluble fraction consists of a saturated hydrocarbon which has not yet been obtained pure. A further 10% consists of a compound containing O. The methyl alcohol-soluble fraction contains a 3rd hydrocarbon which contains 1 double bond reacting with I. The yield of the unsaturated hydrocarbon C45H76 or C50H84 was 29 mgm. per 100 gm. liver.