Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects
Top Cited Papers
- 2 March 2014
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 11 (4), 399-402
- https://doi.org/10.1038/nmeth.2857
Abstract
Bacterial RNA–directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations.Keywords
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