DNA sequence mapping by fluorescence in situ hybridization

Abstract

Various types of DNA probes, such as total genomic DNA, repetitive sequences, unique sequences, and composites of chromosome-specific DNA probes, can be used with fluorescence in situ hybridization (FISH) techniques to address research questions having to do with localization, mapping, and distribution of DNA in situ. FISH involves the formation of a heteroduplex between such DNA probes and chromatin targets on a microscope slide, which can be visualized with fluorescent reporter molecules. Three chromatin targets--metaphase chromosomes, somatic interphases, and zygote interphases--offer increasingly extended states of chromatin which can be strategically selected, individually or in combination, to address specific research questions of interest.