Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.

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Abstract
A porcine genomic clone encoding a major histocompatibility (MHC) antigen was isolated by direct screening of a swine genomic library with a heterologous human MHC c[complementary]DNA probe. Mouse L cells transformed with DNA from the clone stably express swine MHC antigen. Pig alloantisera specifically lyse transformant but not control cell lines in a complement-mediated cytotoxicity assay. Direct immunoprecipitation of radiolabeled cellular protein from transformant lines by pig alloantiserum results in the coprecipitation of swine MHC H chain and mouse .beta.2-microglobulin, demonstrating the association of heterologous subunits of MHC antigens.