Structural features of human leukocyte interferon A as determined by circular dichroism spectroscopy*
- 1 July 1982
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 20 (1), 93-96
- https://doi.org/10.1111/j.1399-3011.1982.tb02658.x
Abstract
The solution structure of human leukocyte (alpha) interferon-A (LeIF-A) purified from E. coli extracts was investigated by circular dichroism spectroscopy. At pH 8.2, the native molecule exhibits 40–70%α-helix and no clearly detectable β-structure. The tertiary structure includes a closely-packed interior containing at least one tryptophan side-chain. Titration to pH 1.5 produces a partial loss of structural features which are rapidly regained on return to neutral pH.Keywords
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