PARTITION CHROMATOGRAPHY OF SUGARS IN WORT AND BEER ON ANION EXCHANGE RESINS

Abstract

Partition chromatography on anion exchange resins in the sulphate form in 85% ethanol at 75°C was used for the separation of monosaccharides in beer and wort. The eluate was analysed automatically by the orcinol method. Simultaneous analysis by a periodate-formaldehyde method permitted determination of glycerol. Oligomers, except for those precipitated by 65% ethanol, were separated and characterized by chromatography in this medium. Sharp separations of disaccharides were made at an intermediate concentration. Species which were not separated on this resin were resolved on a cation exchanger in its lithium form. The identification of oligomers was facilitated by the fact that for oligomers having the same type of glycosidic bonds there exists a straight line relationship between the logarithm of the distribution coefficient and the number of monosaccharide residues.