The secondary structure of bacteriorhodopsin in organic solution
- 22 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 318 (1), 77-79
- https://doi.org/10.1016/0014-5793(93)81331-s
Abstract
Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in Chloroform-methanol (1:1 vv), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of α-helices, reverse turns and β-sheets are similar to the native state. However, the αII-helices, which are the major helical class in native bacteriorhodopsin, are greatly decreased in the solubilized sample. Similarly, the reverse turns and the β-sheets are strongly alteredKeywords
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