SEPARATION AND PARTIAL PURIFICATION OF CENTRAL NERVOUS SYSTEM PEPTIDES FROM LIMULUS POLYPHEMUS WITH HYPERGLYCEMIC AND CHROMATOPHOROTROPIC ACTIVITY IN CRUSTACEANS
Open Access
- 1 February 1978
- journal article
- research article
- Published by University of Chicago Press in The Biological Bulletin
- Vol. 154 (1), 148-156
- https://doi.org/10.2307/1540780
Abstract
Crude acetic acid extracts of the Limulus CNS cause a hyperglycemic response in destalked crayfish (Orconectes immunis) which is dose-dependent. The hyperglycemic factor (LHGF) is stable to boiling, susceptible to oxidation by H2O2 inactivated by pepsin and protease, and has an apparent MW of 6400 daltons as determined on Sephadex G-50. LHGF seems to be a polypeptide. LIrF can be readily separated from another Limulus CNS factor, Limulus-Uca chromatophorotropin (LUC), by gel filtration on Sephadex G-25. Further characterization of LUC indicates that it is a peptide based on its inactivation by numerous proteolytic enzymes and an apparent MW of 1850 daltons. The similarity of these factors to known crustacean hormones is discussed.This publication has 3 references indexed in Scilit:
- Structure of a light-adapting hormone from the shrimp, Pandalus borealisBiochimica et Biophysica Acta (BBA) - Protein Structure, 1976
- Initial characterization and separation of hyperglycemic (diabetogenic) hormone from the crustacean eyestalkGeneral and Comparative Endocrinology, 1967
- UPON THE PRESENCE AND DISTRIBUTION OF A CHROMATOPHOROTROPIC PRINCIPLE IN THE CENTRAL NERVOUS SYSTEM OF LIMULUSThe Biological Bulletin, 1941