Molecularly Cloned Bovine Papillomavirus DNA Transforms Mouse Fibroblasts in Vitro
- 1 March 1983
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 64 (3), 549-557
- https://doi.org/10.1099/0022-1317-64-3-549
Abstract
NIH 3T3 mouse fibroblasts were transformed in vitro by transfection with viral DNA from bovine papillomavirus (BPV) types 4, 2 and 1. The viral DNA were molecularly cloned in pAT153 [Escherichia coli] to construct pBV4, a recombinant plasmid containing the whole genome of BPV4, pBV2 containing the entire genome of BPV2, and pBV1-D1 which contains the 69% transforming DNA fragments of BPV1. The transformed cells lost contact inhibition, were anchorage-independent, required low serum and were tumorigenic in nude mice. This is the first report of cell transformation in vitro by BPV4 DNA. The recombinant plasmids transformed NIH 3T3 cells with an efficiency of 200-300 foci/.mu.g DNA. Cleavage of the recombinant plasmids with enzymes that separate the viral DNA from pAT153 DNA did not significantly alter the efficiency of transformation. In all the transformed cells analyzed, the recombinant plasmids were found as multiple copies of non-integrated monomers.This publication has 17 references indexed in Scilit:
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- A quantitative in vitro focus assay for bovine papilloma virusVirology, 1980
- Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.Proceedings of the National Academy of Sciences, 1979
- Partial characterization of a new type of bovine papilloma virusesVirology, 1979
- In Vitro Transformation by Bovine Papilloma VirusJournal of General Virology, 1979
- Virus-induced papillomas of the alimentary tract of cattleInternational Journal of Cancer, 1978
- Demonstration of two distinct classes of bovine papilloma virusVirology, 1978
- High incidence area of cattle cancer with a possible interaction between an environmental carcinogen and a papilloma virusNature, 1978
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA.Proceedings of the National Academy of Sciences, 1977