Ion Exchange of Lysozyme during Permeation across a Microporous Sulfopropyl‐Group‐Containing Hollow Fiber
- 1 March 1993
- journal article
- research article
- Published by Wiley in Biotechnology Progress
- Vol. 9 (2), 193-198
- https://doi.org/10.1021/bp00020a012
Abstract
A microporous hollow fiber containing a sulfopropyl (SP) group as a strongly acidic cation‐exchange group was prepared by radiation‐induced graft polymerization of glycidyl methacrylate, followed by hydrolysis of the resulting epoxide group into a diol, and then conversion of the diol into the SP group. The SP group density of the resulting hollow fiber ranged from 0.21 to 0.84 mol/kg of dry fiber with a pure water flux of 2.7 m/h at a filtration pressure of 0.1 MPa. Lysozyme adsorption was examined during permeation of the lysozyme solution (pH 6) through the pores across a microporous cation‐exchange hollow fiber. The lysozyme concentration of the effluent penetrating the outside of the hollow fiber did not change irrespective of the residence time of the solution across the hollow fiber, which was indicative of the negligible diffusional resistance of lysozyme to the SP group. The binding capacity of lysozyme to the fiber was constant in this range of SP group density. For comparison, the adsorption characteristics of a cupric chloride solution during permeation were also determined. The binding capacity of Cu to the fiber increased linearly with increasing SP group density, because cupric ions of a smaller size than lysozyme can invade the depths of the grafted polymer branches formed in the amorphous domain of the polymer matrix.Keywords
This publication has 14 references indexed in Scilit:
- Adsorption Characteristics of an Immobilized Metal Affinity MembraneBiotechnology Progress, 1991
- Introduction of a high-density chelating group into a porous membrane without lowering the fluxIndustrial & Engineering Chemistry Research, 1991
- Metal collection using chelating hollow fiber membraneJournal of Membrane Science, 1991
- Water flux and protein adsorption of a hollow fiber modified with hydroxyl groupsJournal of Membrane Science, 1991
- Novel hollow fiber membrane for the removal of metal ion during permeation: preparation by radiation-induced cografting of a crosslinking agent with reactive monomerIndustrial & Engineering Chemistry Research, 1989
- Phosphorylated hollow fibers synthesized by radiation grafting and cross-linkingJournal of Membrane Science, 1989
- Membrane-Based Affinity Technology for Commercial Scale PurificationsNature Biotechnology, 1988
- High-performance ion-exchange chromatography of proteins on non-porous ion exchangersJournal of Chromatography A, 1987
- Reactive polymers XIV. Hydrolysis of the epoxide groups of the copolymer glycidylmethacrylate — ethylenedimethacrylateBritish Polymer Journal, 1977
- Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 Å ResolutionNature, 1965