Biosynthesis of Specific Histones during Meiotic Prophase of Mouse Spermatogenesis 1
- 1 April 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 32 (3), 599-609
- https://doi.org/10.1095/biolreprod32.3.599
Abstract
A kinetics study has demonstrated histone synthesis occurring at two distinct phases during meiotic prophase of mouse spermatogenesis. These two periods have been delineated by quantifying the synthesis of DNA and basic nuclear proteins in spermatogenic cells at discrete intervals following the intratesticular injection of [3H] thymidine and [14C] arginine, respectively. One phase of histone synthesis occurs coincident with DNA synthesis in preleptotene spermatocytes. By contrast, a second phase of histone synthesis occurs during midprophase of meiosis, independent of semiconservative DNA synthesis. The [14C] arginine incorporated into the basic nuclear proteins of pachytene spermatocytes is conserved during spermiogenesis and then subsequently discarded within the residual bodies, which are formed during late spermiogenesis. Fluorographic analyses of isotopically labeled basic nuclear proteins in pachytene spermatocytes has shown that only the somatic complement of histones are synthesized during the preleptotene period, whereas the second phase involves the synthesis of proteins H1t, H2S, and "A". In addition, several nonhistone basic nuclear proteins are synthesized concomitant with the germ cell-specific histones. Thus, the data clearly demonstrate that pachytene spermatocytes actively synthesize a number of novel chromatin-associated polypeptides.This publication has 29 references indexed in Scilit:
- Two-dimensional electrophoretic analysis of major histone species and their variants from somatic and germ-line tissueAnalytical Biochemistry, 1978
- Histones of Rat Testis Chromatin During Early Postnatal Development and Their Interactions with DNA1Biology of Reproduction, 1977
- Noncoincidence of histone and DNA synthesis in cleavage cycles of early development.Proceedings of the National Academy of Sciences, 1977
- Synthesis of histone messenger RNA of HeLa cells during the cell cycleCell, 1977
- Spermatogenic cells of the prepuberal mouse: isolation and morphological characterizationThe Journal of cell biology, 1977
- Histone Synthesis and Replacement during Spermatogenesis in the MouseDifferentiation, 1977
- A cysteine-containing H2B-like histone found in mature mammalian testis.Journal of Biological Chemistry, 1976
- Separation of mouse spermatogenic cells by sedimentation velocityDevelopmental Biology, 1976
- Histones of meiosisExperimental Cell Research, 1967
- The Fluorometric Measurement of Deoxyribonucleic Acid in Animal Tissues with Special Reference to the Central Nervous SystemJournal of Biological Chemistry, 1958