Cell cultures and associated retroviruses in multiple sclerosis
- 1 February 1997
- journal article
- Published by Wiley in Acta Neurologica Scandinavica
- Vol. 95, 22-31
- https://doi.org/10.1111/j.1600-0404.1997.tb08146.x
Abstract
Retroviral particles associated with reverse transcriptase (RT) activity in cell‐cultures from MS patients have been reported by different groups. Cell‐cultures have been used for the study and characterization of the corresponding retroviral genome which we have shown is related to ERV9 in the pol region. Previously unpublished details of a study with monocyte cultures are presented together with observations on leptomeningeal and choroid‐plexus cultures. The generation of self‐transformed cultures after inhibition of interferon, followed by the loss of retroviral expression and recurrent apoptosis, is analyzed. Retroviral particles with RT‐activity are produced in monocyte cultures with an apparent correlation with MS disease activity. However, though leptomeningeal and choroid plexus cells from MS can be passaged for a limited period, their evolution in vitro is not compatible with stable retroviral expression. These culture limitations greatly hampered progress on the elucidation of the retroviral genome sequence.Keywords
This publication has 13 references indexed in Scilit:
- Development of a pan-retrovirus detection system for multiple sclerosis studiesActa Neurologica Scandinavica, 1997
- In vitro transmission and antigenicity of a retrovirus isolated from a multiple sclerosis patientResearch in Virology, 1992
- Just another dubious virus in cells from a patient with multiple sclerosis?The Lancet, 1991
- Isolation of retrovirus from patients with multiple sclerosisThe Lancet, 1991
- Leptomeningeal cell line from multiple sclerosis with reverse transcriptase activity and viral particlesResearch in Virology, 1989
- Pathogenesis of lentivirus infectionsNature, 1986
- Restricted replication of lentiviruses. Visna viruses induce a unique interferon during interaction between lymphocytes and infected macrophages.The Journal of Experimental Medicine, 1985
- Experimental Visna in Icelandic Sheep: The Prototype Lentiviral InfectionClinical Infectious Diseases, 1985
- La barrière hémato-encéphalique. Données morphologiquesLa Revue de Médecine Interne, 1983
- Cultivation of visna virus in tissue cultureArchiv für die gesamte Virusforschung, 1960