Probing Cell‐Type‐Specific Intracellular Nanoscale Barriers Using Size‐Tuned Quantum Dots
- 11 November 2009
- Vol. 5 (22), 2581-2588
- https://doi.org/10.1002/smll.200900744
Abstract
The compartmentalization of size-tuned luminescent semiconductor nanocrystal quantum dots (QDs) in four distinctive cell lines, which would be representative of the most likely environmental exposure routes to nanoparticles in humans, is studied. The cells are fixed and permeabilized prior to the addition of the QDs, thus eliminating any cell-membrane-associated effects due to active QD uptake mechanisms or to specificity of signaling routes in different cell types, but leaving intact the putative physical subcellular barriers. All quantitative assays are performed using a high content analysis (HCA) platform, thereby obtaining robust data on large cell populations. While smaller QDs 2.1 nm in diameter enter the nuclei and localize to the nucleoli in all cell types, the rate and dynamics of their passage vary depending on the cell origin. As the QD size is increased to 4.4 nm, penetration into the cell is reduced but each cell line displays its own cutoff size thresholds reflecting cell-type-determined cytoplasmic and nuclear pore penetration specificity. These results give rise to important considerations regarding the differential compartmentalization and susceptibility of organs, tissues, and cells to nanoparticles, and may be of prime importance for biomedical imaging and drug-delivery research employing nanoparticle-based probes and systems.Keywords
This publication has 40 references indexed in Scilit:
- Nanoparticles target distinct dendritic cell populations according to their sizeEuropean Journal of Immunology, 2008
- Beyond drug deliveryNature Nanotechnology, 2008
- Determining the architectures of macromolecular assembliesNature, 2007
- Nonfunctionalized Nanocrystals Can Exploit a Cell's Active Transport Machinery Delivering Them to Specific Nuclear and Cytoplasmic CompartmentsNano Letters, 2007
- Renal clearance of quantum dotsNature Biotechnology, 2007
- Reversibility in nucleocytoplasmic transportProceedings of the National Academy of Sciences, 2007
- Immunological properties of engineered nanomaterialsNature Nanotechnology, 2007
- Quantum Dots as Cellular ProbesAnnual Review of Biomedical Engineering, 2005
- Quantum dot bioconjugates for imaging, labelling and sensingNature Materials, 2005
- Nuclear Pore Complex Is Able to Transport Macromolecules with Diameters of ∼39 nmMolecular Biology of the Cell, 2002