Evidence for the Role of Subgenomic RNAs in the Production of Potato Virus S coat Protein During in Vitro Translation
- 1 May 1990
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 71 (5), 1247-1249
- https://doi.org/10.1099/0022-1317-71-5-1247
Abstract
Purified virus particles of potato virus S (PVS) when disrupted yielded only one readily resolved band of 7.5 kb in ethidium bromide-stained agarose gels. However Northern hybridization of viral RNA, probed with a clone specific to the viral coat protein gene, revealed a region of subgenomic RNAs of approximately 1.3 kb. Sucrose gradient fractionation of subgenomic RNA revealed that it coded for viral coat protein when translated in vitro in rabbit reticulocyte lysate. Virus particle lengths suggest that this subgenomic RNA may be encapsidated in 100 to 220 nm particles.This publication has 8 references indexed in Scilit:
- Partial Nucleotide Sequence of Potato Virus M RNA Shows Similarities to Potexviruses in Gene Arrangement and the Encoded Amino Acid SequencesJournal of General Virology, 1989
- Organization and Interviral Homologies of the 3'-terminal Portion of Potato Virus S RNAJournal of General Virology, 1989
- Cell-free Translation of Turnip Mosaic Virus RNAJournal of General Virology, 1987
- Characterization of the Coat Protein Subgenomic RNA of White Clover Mosaic VirusJournal of General Virology, 1987
- Nucleotide sequence of the brome mosaic virus genome and its implications for viral replicationJournal of Molecular Biology, 1984
- Narcissus mosaic virus: a potexvirus with an encapsidated subgenomic messenger RNA for coat proteinBioscience Reports, 1983
- Nucleotide sequence of tobacco mosaic virus RNA.Proceedings of the National Academy of Sciences, 1982
- TRANSLATION INVITRO OF RIBONUCLEIC-ACIDS FROM POTATO VIRUS-X, POTATO VIRUS-M AND WHITE CLOVER MOSAIC-VIRUS1981