Pyruvate utilization by synaptosomes is independent of calcium
- 21 April 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 199 (2), 222-226
- https://doi.org/10.1016/0014-5793(86)80484-7
Abstract
The significance of Ca2+ is assessed for the activation of pyruvate oxidation by intact nerve terminals (synaptosomes). Titration of glucose-depleted synaptosomes with pyruvate in the presence of either veratridine or uncoupler stimulates respiration in a Ca2+-independent manner. Additionally, the ability of exogenous pyruvate to support the mitochondrial membrane potential in situ is independent of Ca2+. It is concluded that Ca2+ does not regulate pyruvate oxidation in intact synaptosomes.Keywords
This publication has 19 references indexed in Scilit:
- Relation between mitochondrial calcium transport and control of energy metabolismReviews of physiology, biochemistry and pharmacology, 1985
- Ionophore A23187, Verapamil, Protonophores, and Veratridine Influence the Release of γ‐Aminobutyric Acid from Synaptosomes by Modulation of the Plasma Membrane Potential Rather than the Cytosolic CalciumJournal of Neurochemistry, 1984
- alpha-latrotoxin of black widow spider venom depolarizes the plasma membrane, induces massive calcium influx, and stimulates transmitter release in guinea pig brain synaptosomes.Proceedings of the National Academy of Sciences, 1982
- On the role of the calcium transport cycle in heart and other mammalian mitochondriaFEBS Letters, 1980
- Energy Metabolism in Rat Brain Synaptosomes from Nembutal‐Anesthetized and Nonanesthetized AnimalsJournal of Neurochemistry, 1980
- Energy transduction in intact synaptosomes. Influence of plasma-membrane depolarization on the respiration and membrane potential of internal mitochondria determined in situBiochemical Journal, 1980
- THE CONTROL OF PYRUVATE DEHYDROGENASE IN ISOLATED BRAIN MITOCHONDRIAJournal of Neurochemistry, 1978
- α-Keto acid dehydrogenase complexesArchives of Biochemistry and Biophysics, 1972
- Interconversion and Kinetic Properties of Pyruvate Dehydrogenase from BrainHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1971
- METABOLISM OF GLUCOSE AND GLUTAMATE BY SYNAPTOSOMES FROM MAMMALIAN CEREBRAL CORTEXJournal of Neurochemistry, 1969