Genetic transformation of willows (Salix spp.) byAgrobacterium tumefaciens
- 1 January 1989
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 8 (2), 55-58
- https://doi.org/10.1007/bf00716837
Abstract
Anin vitro transformation method has been developed for stem explants of fast-growing willow clones (Salix spp.) usingAgrobacterium tumefaciens as a vector. Transformants obtained with the strains C58 and GV3101 (pGV3851::pLD1) were selected on hormone-free medium and on medium containing kanamycin, respectively. Transformation was confirmed by Southern blot analysis and nopaline assay. Inoculation of green-house grown plants with nopaline and octopine wildtype strains and shoot or root inducing mutant strains caused undifferentiated tumors at a frequency of 0 to 80%, depending on theSalix genotype and the bacterial strain used.Keywords
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