STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEM
Open Access
- 1 October 1951
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 94 (4), 305-322
- https://doi.org/10.1084/jem.94.4.305
Abstract
Evidence has been presented that influenza viruses both of type A and B partially inactivated by ultraviolet irradiation may regain their capacity to propagate in the allantoic membrane of the chick embryo. In using such irradiated preparations as inocula for growth curve experiments it could be shown that the development of hemagglutinins as well as of infectivity preceded at rates resembling those noted with more than 10 times the amount of infective virus actually found in the irradiated seed. Partial inactivation of the inocula by heating to 56°C. gave similar results. The phenomenon was observed only with seed irradiated for short periods of time so that the virus particles sustained only few hits of radiation. On prolonged exposure resulting in numerous hits per virus particle the capacity of reactivation was lost. Likewise, an irradiated preparation capable of reactivation in the allantoic membrane, could not be diluted more than about 30-fold and still clearly produce this phenomenon. This indicated that reactivation is obtained only when one host cell adsorbs more than one non-infective virus particle but not upon adsorption of a single particle. These data are in striking agreement with the phenomenon of "multiplicity reactivation" observed in the bacteriophage-E. coli system by Luria and Dulbecco.Keywords
This publication has 20 references indexed in Scilit:
- STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEMThe Journal of Experimental Medicine, 1951
- STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEMThe Journal of Experimental Medicine, 1951
- Incomplete Growth Cycle of Influenza Virus in Mouse BrainExperimental Biology and Medicine, 1950
- Interference Phenomena Between Animal Viruses: A ReviewThe Journal of Immunology, 1950
- STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEMThe Journal of Experimental Medicine, 1949
- STUDIES ON HOST-VIRUS INTERACTIONS IN THE CHICK EMBRYO-INFLUENZA VIRUS SYSTEMThe Journal of Experimental Medicine, 1949
- ONE-STEP GROWTH CURVES OF VARIOUS STRAINS OF INFLUENZA A AND B VIRUSES AND THEIR INHIBITION BY INACTIVATED VIRUS OF THE HOMOLOGOUS TYPEThe Journal of Experimental Medicine, 1949
- REACTIONS BETWEEN INFLUENZA VIRUS AND A COMPONENT OF ALLANTOIC FLUIDThe Journal of Experimental Medicine, 1948
- THE GROWTH CYCLE OF INFLUENZA VIRUS-A - A STUDY OF THE RELATIONS BETWEEN VIRUS, SOLUBLE ANTIGEN AND HOST CELL IN FERTILE EGGS INOCULATED WITH INFLUENZA VIRUS1948
- Reactivation of Irradiated Bacteriophage by Transfer of Self-Reproducing UnitsProceedings of the National Academy of Sciences, 1947