THE USE OF ION-EXCHANGE MATERIALS IN THE FRACTIONATION OF GONADOTROPHINS FROM URINE
- 1 December 1959
- journal article
- research article
- Published by Bioscientifica in Acta Endocrinologica
- Vol. XXXII (IV), 509-518
- https://doi.org/10.1530/acta.0.xxxii0509
Abstract
Urine was collected from postmenopausal women and normal men, and extracted usually within 24 hours, using the benzoic-tungstic acid method extraction and diethylamino ethyl cellulose fractionation. The mouse uterine weight assay was used to measure the combined effect of follicle stimulating hormone (FSH) and interstitial cell stimulating hormone (ICSH), while the mouse ovarian weight method was used for FSH. The fractions from postmenopausal women were 23 times more potent than the standard human menopausal gonadotropin (HMG) 20A. There were no differences in the relative amounts of FSH or ICSH in the postmenopausal urine as compared to the standard, but in the male there was a greater ratio of FSH to ICSH in the eluted fraction and a greater ratio of ICSH to FSH in the absorbed fraction.Keywords
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