1H COSY spectra of superfused brain slices of rat: Ex vivo direct assignment of resonances

Abstract
Realization of 1 D 1H spectra of superfused brain slices of rats is described. Two‐dimensional 1H− 1H COSY spectra have been used for the direct assignment of resonances on living tissue (lactate, GABA, N‐acetyl aspartate, aspartate, glutamine/glutamate, creatine/phosphocreatine, taurine, inositol, choline/ethanolamine). Utilization of the SUPERCOSY sequence permits simultaneously the enhancement of off‐diagonal signals for small metabolites and the reduction of the signals for water and macromolecules.