The bentonite granuloma. Characterization of a model system for infectious and foreign body granulomatous inflammation using soluble mycobacterial, histoplasma and schistosoma antigens.
- 1 March 1973
- journal article
- Vol. 24 (3), 511-29
Abstract
A model for the study of granulomatous inflammation has been developed, employing bentonite particles (65 μm in diameter) coated with soluble antigens derived from Mycobacterium tuberculosis, Histoplasma capsulatum and Schistosoma mansoni. These particles are injected i v. into the micro-vasculature of the lungs of unsensitized mice or mice sensitized to the above antigens in a variety of ways. Uncoated particles or those coated with heterologous antigens elicit rapidly forming small foci of inflammatory cells composed mainly of macrophages. In contrast, particles coated with homologous antigens elicit large hypersensitivity-type granulomas composed of lymphocytes, macrophages, eosinophils and occasionally epithelioid cells. The occurrence of hypersensitivity-type granulomas correlates well with delayed footpad swelling also elicited by homologous antigen. In addition, the florid specific granulomatous inflammation is transferrable to syngeneic recipients with immune lymphoid cells but not with antiserum. Finally, when bare or heterologous antigen-coated particles are injected into sensitized mice which are simultaneously injected i.v. with homologous antigen, hypersensitivity granulomas appear around the particles. This bentonite model thus facilitates the study of both the foreign body and hypersensitivity granulomas and suggests mechanisms by which they might be formed.This publication has 23 references indexed in Scilit:
- The Role of Phospholipids in Schistosome GranulomasThe Journal of Infectious Diseases, 1971
- In Vitro Approaches to the Mechanism of Cell-Mediated Immune ReactionsAdvances in Immunology, 1971
- Specific Granulomatous Hypersensitivity elicited by Bentonite Particles coated with Soluble Antigens from Schistosome Eggs and Tubercle BacilliNature, 1971
- IN VITRO AND IN VIVO ACTIVITY OF A LYMPHOCYTE AND IMMUNE COMPLEX-DEPENDENT CHEMOTACTIC FACTOR FOR EOSINOPHILSThe Journal of Experimental Medicine, 1971
- MECHANISM OF EOSINOPHILIAThe Journal of Experimental Medicine, 1970
- THE ROLE OF CHEMICAL MEDIATORS IN THE INFLAMMATORY RESPONSE INDUCED BY FOREIGN BODIES: COMPARISON WITH THE SCHISTOSOME EGG GRANULOMAThe Journal of Experimental Medicine, 1970
- THE INFLUENCE OF IMMUNOLOGICALLY COMMITTED LYMPHOID CELLS ON MACROPHAGE ACTIVITY IN VIVOThe Journal of Experimental Medicine, 1969
- Leukotactic Factor Produced by Sensitized LymphocytesScience, 1969
- Mechanism of a Reaction in Vitro Associated with Delayed-Type HypersensitivityScience, 1966
- THE ADSORPTION OF PROTEINS ON ERYTHROCYTES TREATED WITH TANNIC ACID AND SUBSEQUENT HEMAGGLUTINATION BY ANTIPROTEIN SERAThe Journal of Experimental Medicine, 1951