Detection of avocado sunblotch viroid in chloroplasts of avocado leaves by in situ hybridization
- 1 September 1994
- journal article
- Published by Springer Nature in Archiv für die gesamte Virusforschung
- Vol. 138 (3-4), 385-390
- https://doi.org/10.1007/bf01379142
Abstract
In situ hybridization experiments were carried out to detect avocado sunblotch viroid (ASBVd) in foliar tissue of avocado, using a digoxigeninlabelled RNA probe complementary to the ASBVd-RNA in sections of aldehyde-fixed, LRGold-embedded leaf samples. Detection of the probe was made through anti-digoxigenin antibody and protein-A colloidal gold (20 nm). Seventy to 80% of the signals came from chloroplast while the cytoplasm and vacuole were labelled with ca. 10% of the gold particles. This is in contrast with the subcellular localization of potato spindle tuber viroid and some other related viroids, which are mainly found in the nucleus.Keywords
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