Peptidyl puromycin synthesis; effect of several antibiotics which act on 50 S ribosomal subunits

Abstract

The effects of several antibiotics which are know to bind with 50 S ribosomal subunits, on the formation of several di- and tri-peptidyl puromycins have been examined. Tylosin and spiramycin inhibited the formation of phenylalanyl-¹⁴C-phenylalanyl-puromycin, glycyl-¹⁴C-phenyllalanyl-puromycin, leucyl-¹⁴C-phenylalanyl-puromycin, N ^ϵ-carbobenzoxylysyl-¹⁴C-phenylalanyl-puromycin, and valyl-glycyl-¹⁴C-phenylalanyl-puromycin as well as N-acetyl-¹⁴C-phenylalanyl-puromycin. Of these compounds, erythromycin and oleandomycin selectively inhibited the formation of phenylalanyl-¹⁴C-phenylalanyl-puromycin. Although chloramphenicol and lincomycin inhibited the formation of most of these peptidyl puromycins, the formation of phenylalanyl-¹⁴C-phenylalanyl-puromycin and leucyl-¹⁴C-phenylalanyl-puromycin was found to be resistant to these antibiotics. So far, no significant effect of siomycin has been observed on pepetidyl puromycin formation in the absence of G factor.