Determination of the Amino‐Acid Sequence of the Ribosomal Protein S8 of Escherichia coli
- 28 June 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 66 (1), 49-56
- https://doi.org/10.1111/j.1432-1033.1976.tb10424.x
Abstract
The primary structure of protein S8 from the 30S ribosomal subunit of E. coli was determined mainly by automatic Edman degradation using a modified Beckman protein sequenator and the solid-phase sequenator of Laursen. The complete sequence, containing 109 amino acids, was derived by analysing peptides from tryptic, chymotryptic, thermolysin, staphylococcal protease and cyanogen bromide digestion of the protein. The amino acid composition was (aspartic acid)6, (asparagine)3, (threonine)5, (serine)5, (glutamic acid)7, (glutamine)6, (proline)5, (glycine)6, (alanine)11, (valine)9, (methionine)4, (isoleucine)7, (leucine)9, (tyrosine)3, (phenylalanine)3, (lysine)11, (arginine)8, (cysteine)1. S8 is a basic protein and binds to the 16S RNA: knowledge of its sequence is necessary for a detailed study of its interaction with the rRNA.This publication has 23 references indexed in Scilit:
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