Effect of verapamil on 45Ca uptake by synaptosomes.
- 1 January 1980
- journal article
- research article
- Published by Elsevier in The Japanese Journal of Pharmacology
- Vol. 30 (2), 207-211
- https://doi.org/10.1254/jjp.30.207
Abstract
The effect of verapamil on K-stimulated 45Ca uptake (the difference between uptake in 60 mM-Ko medium and 5 mM-Ko medium) was studied using a synaptosomal fraction from rat brain. Verapamil inhibited K-stimulated 45Ca uptake, but not 45Ca uptake in 5 mM-Ko media (1 mM- and 0.1 mM-Cao media). The concentrations of verapamil inducing 50% inhibition of K-stimulated 45Ca uptake (ID50) in 1 mM- and 0.1 mM-Cao media were not significantly different, being .apprx. 10-4 M and 2 .times. 10-4 M, respectively. Like verapamil, Mn2+ inhibited only K-stimulated 45Ca uptake, but its ID50 values in 1 mM- and 0.1 mM-Cao media were .apprx. 1.7 mM and 0.2 mM, respectively. Verapamil and Mn3+ specifically inhibit K-stimulated 45Ca uptake, but that the modalities of their inhibitory effects on K-stimulated 45Ca uptake are different.This publication has 3 references indexed in Scilit:
- Bound Forms of Ca Taken Up by the Synaptic Plasma MembraneThe Japanese Journal of Pharmacology, 1976
- ISOLATION OF NERVE ENDINGS FROM BRAIN - AN ELECTRON-MICROSCOPIC STUDY OF CELL FRAGMENTS DERIVED BY HOMOGENIZATION AND CENTRIFUGATION1962
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951