Radioreceptor assay of methionine-enkephalin-like substance in human cerebrospinal fluid
- 1 August 1980
- journal article
- research article
- Published by Wolters Kluwer Health in Pain
- Vol. 9 (1), 63-72
- https://doi.org/10.1016/0304-3959(80)90029-9
Abstract
A sensitive and simple radioreceptor assay system for measuring methionine(met)-enkephalin-like substance in human CSF was developed using a particulate fraction of rat brain as a receptor preparation and [3H]dihydromorphine as a radiolabeled ligand in the presence of 1 mM EDTA and 2 mM magnesium acetate. Metenkephalin-like substance was purified from CSF by the combination of Sephadex G-10 and SP-Sephadex (H+) column chromatographies free of Na and large MW substances such as .beta.-endorphin. Assays were performed on samples obtained from normal subjects and patients with disease of the brain or pituitary by lumbar or ventricular puncture. The mean level in samples obtained from normal subjects by lumbar puncture was 2.6 .+-. 1.0 pmol/ml.This publication has 15 references indexed in Scilit:
- ACUPUNCTURE IN HEROIN ADDICTS: CHANGES IN MET-ENKEPHALIN AND $beta;-ENDORPHIN IN BLOOD AND CEREBROSPINAL FLUIDThe Lancet, 1979
- Enkephalin-Like Material Elevated in Ventricular Cerebrospinal Fluid of Pain Patients After Analgetic Focal StimulationScience, 1978
- Enkephalin-like material in normal human CSF: Measurement and levelsLife Sciences, 1978
- Behavioral Neurochemistry: Neuroregulators and Behavioral StatesScience, 1978
- Nonhomogeneous distribution of 5-hydroxyindoleacetic acid and homovanillic acid in the lumbar cerebrospinal fluid of manJournal of the Neurological Sciences, 1977
- Binding of 3H-leu-enkephalin in rat striatum: Partial inhibition by morphine or naloxoneEuropean Journal of Pharmacology, 1977
- Immunohistochemical studies using antibodies to leucine-enkephalin: Initial observations on the nervous system of the ratNeuroscience, 1976
- C fragment of lipotropin has a high affinity for brain opiate receptorsNature, 1976
- Isolation of an endogenous compound from the brain with pharmacological properties similar to morphineBrain Research, 1975
- Measurement of acid monoamine metabolites in human and animal cerebrospinal fluidJournal of Neurochemistry, 1968