Studies on the Interaction of Ca2+Ions with Some Fractions of the Neurospecific S‐100 Protein
- 1 June 1981
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 36 (6), 1904-1915
- https://doi.org/10.1111/j.1471-4159.1981.tb10814.x
Abstract
Fractions of neurospecific S-100 protein were purified from bovine brain and their physicochemical properties were studied. Conformational changes caused by the binding of calcium to S-100 protein fractions were detected by means of differential and fluorescence spectroscopy. Fractions demonstrating opposite shifts of their spectra also differ in the distribution in double-phase system. The number of calcium-binding centers and their association constants were determined by means of equilibrium dialysis and gel filtration. The nature of the differences in the interaction of various S-100 protein fractions with calcium is discussed.Keywords
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