Growth of Mouse Ascites Tumor Cells in Diffusion Chambers. I. Studies of Growth Rate of Cells and of the Rate of Entry of Antibody2

Abstract

Sampling chambers, designed to permit the flow of ascites fluid but to exclude host cells, will admit isoantibody. Hemagglutinating, neutralizing, and protective effects have been demonstrated. The rate of fluid entry into cell-impermeable millipore chambers is slow, and the membrane acts as a considerable diffusion barrier to the entry of antibody. The peritoneum acts as an initial barrier to the flow of antibody from the blood stream to the chamber. Ascites tumor cells grow progressively in the sampling chamber after a sharp, early drop in cell number. The rate of rapid growth is sufficiently consistent to permit quantitative measurements of the effect of antibody.