Removal of psoralen monoadducts and crosslinks by human cell free extracts
Open Access
- 11 September 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 19 (17), 4623-4629
- https://doi.org/10.1093/nar/19.17.4623
Abstract
Human cell free extracts are capable of carrying out damage-induced DNA synthesis in response to DNA damage by UV, psoralen, and cisplatin. We show that this damage-induced DNA synthesis is associated with removal of psoralen adducts and therefore is ‘repair synthesis’ and not an aberrant DNA synthesis reaction potentiated by DNA deformed by adducts. By comparing the denaturable fraction of psoralen adducted DNA which becomes labeled in the repair reaction to that of terminally labeled DNA (without repair) we have found that all DNA synthesis induced by psoralen monoadducts is the consequence of removal of these adducts. By the same approach we have obtained preliminary evidence that this in vitro system is capable of removing psoralen crosslinks as well.Keywords
This publication has 21 references indexed in Scilit:
- ATP-dependent partitioning of the DNA template into supercoiled domains by Escherichia coli UvrAB.Proceedings of the National Academy of Sciences, 1991
- RecA-dependent incision of psoralen-crosslinked DNA by (A)BC excinucleaseNucleic Acids Research, 1991
- Effect of the antitumor drug cis-diamminedichloroplatinum(II) and related platinum complexes on eukaryotic DNA replicationBiochemistry, 1990
- Substrate overlap and functional competition between human nucleotide excision repair and Escherichia coli photolyase and (A)BC excision nucleaseBiochemistry, 1990
- Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extractsCell, 1988
- Processing of psoralen adducts in an active human gene: Repair and replication of DNA containing monoadducts and interstrand cross-linksCell, 1987
- Wavelength dependence for the photoreversal of a psoralen-DNA crosslinkBiochemistry, 1986
- Photochemistry of the furan-side 8-methoxypsoralen-thymidine monoadduct inside the DNA helix. Conversion to diadduct and to pyrone-side monoadductBiochemistry, 1985
- The psoralen-DNA photoreaction. Characterization of the monoaddition products from 8-methoxypsoralen and 4,5',8-trimethylpsoralenBiochemistry, 1982
- Photochemical production of psoralen - DNA monoadducts capable of subsequent photocrosslinkingNucleic Acids Research, 1978