Quantitative Determination of Griseofulvin by a Spectrophotofluorometric Assay

Abstract

The present use of griseofulvin as a systemic antifungal agent demands a simple but accurate assay of the drug.¹ The best technique reported is the excellent spectrophotofluorometric method developed by Bedford, Child, and Tomich in England.² This extremely sensitive quantitative procedure depends upon the fluorescent properties of griseofulvin after its extraction from serum. The findings of Bedford et al. show that the fluorescence increases linearly with concentration over a range of 0.05μg.0.5μg. per milliliter. We have confirmed the utility of the assay method and describe again the procedure, stressing points which we found troublesome. Serum levels of griseofulvin are reported in persons who have taken the drug for an extended period of time, as compared with others who have had only a single dose. Method The instrument used in the present study was the Aminco-Bowman Spectrophotofluorometer, whereas Bedford et al. used a Farrand Spectrophotofluorometer. Radiation from a xenon