Murine Coronaviruses Encoding nsp2 at Different Genomic Loci Have Altered Replication, Protein Expression, and Localization
- 1 December 2008
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 82 (23), 11964-11969
- https://doi.org/10.1128/jvi.01126-07
Abstract
Partial or complete deletion of several coronavirus nonstructural proteins (nsps), including open reading frame 1a (ORF1a)-encoded nsp2, results in viable mutant proteins with specific replication defects. It is not known whether expression of nsps from alternate locations in the genome can complement replication defects. In this report, we show that the murine hepatitis virus nsp2 sequence was tolerated in ORF1b with an in-frame insertion between nsp13 and nsp14 and in place of ORF4. Alternate encoding or duplication of the nsp2 gene sequence resulted in differences in nsp2 expression, processing, and localization, was neutral or detrimental to replication, and did not complement an ORF1a Delta nsp2 replication defect. The results suggest that wild-type genomic organization and expression of nsps are required for optimal replication.Keywords
This publication has 39 references indexed in Scilit:
- A Novel Mutation in Murine Hepatitis Virus nsp5, the Viral 3C-Like Proteinase, Causes Temperature-Sensitive Defects in Viral Growth and Protein ProcessingJournal of Virology, 2008
- Mouse Hepatitis Coronavirus RNA Replication Depends on GBF1-Mediated ARF1 ActivationPLoS Pathogens, 2008
- Localization and Membrane Topology of Coronavirus Nonstructural Protein 4: Involvement of the Early Secretory Pathway in ReplicationJournal of Virology, 2007
- Replication of Murine Hepatitis Virus Is Regulated by Papain-Like Proteinase 1 Processing of Nonstructural Proteins 1, 2, and 3Journal of Virology, 2006
- Crystal structure and mechanistic determinants of SARS coronavirus nonstructural protein 15 define an endoribonuclease familyProceedings of the National Academy of Sciences, 2006
- The nsp2 Replicase Proteins of Murine Hepatitis Virus and Severe Acute Respiratory Syndrome Coronavirus Are Dispensable for Viral ReplicationJournal of Virology, 2005
- Coronaviruses as Vectors: Stability of Foreign Gene ExpressionJournal of Virology, 2005
- Cleavage between Replicase Proteins p28 and p65 of Mouse Hepatitis Virus Is Not Required for Virus ReplicationJournal of Virology, 2004
- Unique and Conserved Features of Genome and Proteome of SARS-coronavirus, an Early Split-off From the Coronavirus Group 2 LineageJournal of Molecular Biology, 2003
- The Autocatalytic Release of a Putative RNA Virus Transcription Factor from Its Polyprotein Precursor Involves Two Paralogous Papain-like Proteases That Cleave the Same Peptide BondPublished by Elsevier ,2001