SPECTROPHOTOMETRIC DETERMINATIONS OF BILIRUBIN

Abstract

Study of precautions to be observed in the use of the spectrophotometer for the determination of bilirubin. Effects of fading and turbidity of solutions and errors due to hemolysis of blood cells are discussed. The laws of Lambert and Beer apply to the transmission of light if care is taken to avoid fading of the solutions. Bilirubin can be detected by spectro-photometric methods in a concentration of 1/50 of that detectable by the Van den Bergh test.