Cloning of a full‐length complementary DNA for fatty‐acid‐binding protein from bovine heart
Open Access
- 1 August 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 175 (3), 549-556
- https://doi.org/10.1111/j.1432-1033.1988.tb14227.x
Abstract
A full-length cDNA for bovine heart fatty-acid-binding protein (H-FABP) was cloned from a γgt11 cDNA library established from bovine heart muscle. The cDNA sequence shows an open reading frame coding for a protein with 133 amino acids. Colinearity with the amino acid sequences of four tryptic peptides was asserted. H-FABP isolated from bovine heart begins with an N-acetylated valine residue, however, as derived from analysis of the tryptic, amino-terminal-blocked peptide and the molecular mass of the peptide obtained via secondary-ion mass spectrometry. The molecular mass of the total protein is 14673 Da. Bovine H-FABP is 89% homologous to rat H-FABP and 97% homologous to the bovine mammary-derived growth-inhibition factor described recently by Böhmer et al. [J. Biol. Chem. 262, 15137–15143 (1987)l. Significant homologies were also found with bovine myelin protein P2 and murine adipocyte protein p422. Secondary-structure predictions were proposed for these proteins, based on computer analysis, which reveal striking similarities.This publication has 41 references indexed in Scilit:
- Antibodies against mammary derived growth inhibitor (MDGI) react with a fibroblast growth inhibitor and with heart fatty acid binding proteinBiochemical and Biophysical Research Communications, 1987
- Tissue expression of three structurally different fatty acid binding proteins from rat heart muscle, liver, and intestineBiochemical and Biophysical Research Communications, 1986
- A time-of-flight mass spectrometer for static SIMS applicationsJournal of Vacuum Science & Technology A, 1985
- Detection of subnanogram quantities of DNA and RNA on native and denaturing polyacrylamide and agarose gels by silver stainingElectrophoresis, 1983
- A simple method for displaying the hydropathic character of a proteinJournal of Molecular Biology, 1982
- Micro‐sequence analysis of peptides and proteins using 4‐NN‐dimethylaminoazobenzene 4′‐isothiocyanate/phenylisothiocyanate double coupling methodFEBS Letters, 1978
- Triplet information in helix prediction applied to the analysis of super-secondary structuresJournal of Molecular Biology, 1977
- Conformational properties of amino acid residues in globular proteinsJournal of Molecular Biology, 1976
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970