The role of GTP in prostaglandin E1 stimulation of adenylate cyclase in platelet membranes
- 15 July 1983
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 214 (1), 231-234
- https://doi.org/10.1042/bj2140231
Abstract
Adenylate cyclase activity in platelet membrane preparations was measured in the presence of prostaglandin E1 (PGE1), GTP and a non-hydrolyzable analog of GDP, guanosine 5''-[.beta.-thio]diphosphate (GDP[.beta.S]). A dose-dependent inhibition of adenylate cyclase by GDP[.beta.S] was observed that could be reversed either by adding increased amounts of GTP or of PGE1.This publication has 15 references indexed in Scilit:
- Biochemical Properties of Hormone-Sensitive Adenylate CyclaseAnnual Review of Biochemistry, 1980
- Properties of a factor in cytosol that enhances hormones-stimulated adenylate cyclase activityBiochemical Journal, 1980
- Regulation of human platelet adenylate cyclase by epinephrine, prostaglandin E1, and guanine nucleotides. Evidence for separate guanine nucleotide sites mediating stimulation and inhibition.Journal of Biological Chemistry, 1979
- Guanosine 5'-O-(2-thiodiphosphate). An inhibitor of adenylate cyclase stimulation by guanine nucleotides and fluoride ions.Journal of Biological Chemistry, 1979
- Activation of rat liver plasma membrane adenylate cyclase by a cytoplasmic protein factorFEBS Letters, 1977
- Platelet plasma membrane glycoproteins. Evidence for the presence of nonequivalent disulfide bonds using nonreduced-reduced two-dimensional gel electrophoresis.Journal of Biological Chemistry, 1977
- A simple enzymic method for the synthesis of adenosine 5′-[α-32P]triphosphate on a preparative scaleBiochemical Journal, 1977
- Activation by GTP of liver adenylate cyclase in the presence of high concentrations of ATPBiochemical and Biophysical Research Communications, 1976
- A highly sensitive adenylate cyclase assayAnalytical Biochemistry, 1974
- Requirement for Guanosine Triphosphate in the Prostaglandin Activation of Adenylate Cyclase of Platelet MembranesJournal of Biological Chemistry, 1972